Application
WB analysis of extracts from various cell lines using DDX5 antibody.Application
ICC/IF analysis of HeLa cells using DDX5 antibody (green) and DAPI (blue).Application
ICC/IF analysis of U20S cell using DDX5 antibody (red) and DAPI (blue).Application
IHC-P analysis of rat liver tissue using DDX5 antibody at a dilution of 1:200.Application
IHC-P analysis of rat kidney tissue using DDX5 antibody at a dilution of 1:200.Application
IHC-P analysis of human thyroid cancer using DDX5 antibody at a dilution of 1:200.| Product Name | DDX5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DDX5DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human DDX5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HUMP68 , DEAD (Asp-Glu-Ala-Asp) box helicase 5 , p68 , G17P1 , HLR1 |
|---|---|
| Target | DDX5 |
| Molecular Weight(MW) | 69 kDa (note) |
Application
WB analysis of extracts from various cell lines using DDX5 antibody.
Application
ICC/IF analysis of HeLa cells using DDX5 antibody (green) and DAPI (blue).
Application
ICC/IF analysis of U20S cell using DDX5 antibody (red) and DAPI (blue).
Application
IHC-P analysis of rat liver tissue using DDX5 antibody at a dilution of 1:200.
Application
IHC-P analysis of rat kidney tissue using DDX5 antibody at a dilution of 1:200.
Application
IHC-P analysis of human thyroid cancer using DDX5 antibody at a dilution of 1:200.| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
