Application
Immunohistochemistry of RIG-1 in human heart tissue with RIG-1 antibody at 10 ug/ml.Application
Western blot analysis of RIG-1 in C2C12 cell lysate with RIG-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.| Product Name | DDX58 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to RIG-1The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG-1) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG-1 activates the kinases TBK1 and IKKepsilon through the adaptor protein IPS-1. These kinases then phosphorylate the transcription factors IRF-3 and IRF-7 which are essential for the expression of type-I interferons. RIG-1 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus. |
| Immunogen | the immunogen is a recombinant protein from 201-713 of human RIG1 |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| WB:1:500~1:2000 IHC-P:1:50~1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Dead box protein 58 Dead box protein58 RIG-I RIG-1 ddx58 RIG1 RIGI Retinoic acid inducible gene 1 protein Retinoic acid inducible gene1 protein RIG I RIG 1 |
|---|---|
| Target | RIG-1 |
| Tissue Specificity | This antibody is specific for RIG-1 |
Application
Immunohistochemistry of RIG-1 in human heart tissue with RIG-1 antibody at 10 ug/ml.
Application
Western blot analysis of RIG-1 in C2C12 cell lysate with RIG-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.| Positive Control | Lysates: Cat. No. GTX27182 - C2C12 Cell LysateCat. No. GTX27916 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC-P:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
