Application
DDX6 antibody detects DDX6 protein at cytoplasm on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. DDX6 antibody diluted at 1:500.Application
DDX6 antibody detects DDX6 protein at cytoplasm and P body by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDX6 protein stained by DDX6 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE DDX6 antibody diluted at 1:1000| Product Name | DDX6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DDX6 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 6)This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DDX6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chicken, Bovine, Guinea pigRat (98%), Xenopus laevis (94%), Chicken (99%), Bovine (100%), Guinea pig (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ36338 antibody, HLR2 antibody, P54 antibody, RCK antibody, DDX6 antibody, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD) antibody, DEAD box protein 6 antibody, probable ATP-dependent RNA helicase DDX6 antibody, DEAD box-6 antibody, |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) (2) |
| Cellular Localization | Cytoplasm , P-body |
Application
DDX6 antibody detects DDX6 protein at cytoplasm on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. DDX6 antibody diluted at 1:500.
Application
DDX6 antibody detects DDX6 protein at cytoplasm and P body by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDX6 protein stained by DDX6 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE DDX6 antibody diluted at 1:1000| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
