Application
Immunohistochemical analysis of paraffin-embedded TOV112D xenograft, using Decorin antibody at 1:100 dilution.Application
Immunofluorescence analysis of methanol-fixed A549, using Decorin antibody at 1:200 dilution.Application
Decorin antibody detects DCN protein by western blot analysis. A. 30 µg A549 whole cell lysate/extract B. 30 µg H1299 whole cell lysate/extract 10% SDS-PAGE Decorin antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Decorin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Decorin (decorin)The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Decorin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Rabbit, Sheep, Chimpanzee, Bovine, Guinea pigMouse (82%), Rat (80%), Dog (93%), Pig (89%), Rabbit (91%), Sheep (89%), Chimpanzee (100%), Bovine (89%), Guinea pig (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CSCD antibody DSPG2 antibody PG40 antibody PGII antibody PGS2 antibody SLRR1B antibody DCN antibody PG-S2 antibody decorin proteoglycan antibody proteoglycan core protein antibody bone proteoglycan II antibody dermatan sulphate proteoglycans II |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (2) |
| Cellular Localization | Secreted , extracellular space , extracellular matrix |
Application
Immunohistochemical analysis of paraffin-embedded TOV112D xenograft, using Decorin antibody at 1:100 dilution.
Application
Immunofluorescence analysis of methanol-fixed A549, using Decorin antibody at 1:200 dilution.
Application
Decorin antibody detects DCN protein by western blot analysis. A. 30 µg A549 whole cell lysate/extract B. 30 µg H1299 whole cell lysate/extract 10% SDS-PAGE Decorin antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A549 , H1299 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
