Application
DEDD antibody detects DEDD protein at nucleus by immunofluorescent analysis. Sample: NT2/D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DEDD protein stained by DEDD antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | DEDD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DEDD (death effector domain containing)This gene encodes a protein that contains a death effector domain (DED). DED is a protein-protein interaction domain shared by adaptors, regulators and executors of the programmed cell death pathway. Overexpression of this gene was shown to induce weak apoptosis. Upon stimulation, this protein was found to translocate from cytoplasm to nucleus and colocalize with UBTF, a basal factor required for RNA polymerase I transcription, in the nucleolus. At least three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DEDD. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Rhesus Monkey, BovineMouse (98%), Rat (98%), Xenopus laevis (86%), Rhesus Monkey (99%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KE05 , DEFT , FLDED1 , DEDD1 , CASP8IP1 , death effector domain containing |
|---|---|
| Target | DEDD |
| Molecular Weight(MW) | 37 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus , nucleolus |
Application
DEDD antibody detects DEDD protein at nucleus by immunofluorescent analysis. Sample: NT2/D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DEDD protein stained by DEDD antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
