Application
DEK antibody detects DEK protein by western blot analysis. MCF-7 whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DEK antibody diluted at 1:1000.| Product Name | DEK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DEKThis gene encodes a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA, and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this region, increased expression of this gene, and the presence of antibodies against this protein are all associated with various diseases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus of human DEK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, Rhesus Monkey, Bovine, Guinea pigMouse (92%), Rat (92%), Chicken (84%), Rhesus Monkey (100%), Bovine (92%), Guinea pig (92%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DEK oncogene , D6S231E |
|---|---|
| Target | DEK |
| Molecular Weight(MW) | 43 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
DEK antibody detects DEK protein by western blot analysis. MCF-7 whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DEK antibody diluted at 1:1000.| Positive Control | MCF-7 , MCF-7 nuclear extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
