Rabbit polyclonal antibody to delta Opioid receptorThe opioid receptors are G-protein coupled, seven-transmembrane domain receptors for enkephalins, endorphins, and dynorphins. Three different opioid receptor subtypes (kappa , delta, and mu) were first identified by their different selectivities for various naturally occurring alkaloid opioid ligands, and subsequently confirmed by molecular cloning. The amino acid sequences of the opioid receptor subtypes are ~70% homologous, and are similar to somatostatin receptors (SSTRs) showing ~40 % homology with SSTR1. G-protein binding is thought to occur at the third intracellular loop of the opioid receptors, which is also the location of consensus sequences for phosphorylation of the receptor. Interestingly, the genes encoding the specific receptor subtypes are found on different chromosomes in both the human and mouse genomes.
Immunogen
It was a synthetic peptide, corresponding to amino acids 320-370 of Human DOR-1.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHCWB
We recommend the following starting dilutions: Western Blot 1:500-1:1000, immunohistochemistry 1:50-1:200. Optimal concentration should be determined experimentally by the end user.
Species Reactivity
HumanMouseRat
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
OPRD1, 24613, OPRD, 4985, 18386
Target
delta Opioid receptor
Tissue Specificity
DOR-1 (R356) detects endogenous levels of DOR-1 protein.
Application
Application
Western Blot: Delta Opioid Receptor Antibody [ - (WB) analysis of DOR-1 (R356) antibody in extracts from HUVEC cells.
Application Notes
We recommend the following starting dilutions: Western Blot 1:500-1:1000, immunohistochemistry 1:50-1:200. Optimal concentration should be determined experimentally by the end user.
Additional Information
Form
Liquid
Storage Instructions
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Storage Buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.