Application
Sample (20 µg of whole cell lysate) A: BHK-21 B: Dengue virus 2 infect BHK-21 C: JEV infect BHK-21 7.5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody diluted at 1:2000. Blue: Hoechst 33342 staining.Application
Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS3 (Dengue virus 2) antibody at 1:2000 dilution.Application
NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein by western blot analysis. A. 30 µg BHK-21 whole cell extract B. 30 µg whole cell extract of Dengue virus type 1 infected BHK-21 cells C. 30 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells D. 30 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells E. 30 µg whole cell extract of Dengue virus type 4 infected BHK-21 cells 7.5% SDS-PAGE NS3 (Dengue virus) antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody diluted at 1:2000. Blue: Hoechst 33342 staining.Application
NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody diluted at 1:2000. Blue: Hoechst 33342 staining.Application
NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody diluted at 1:2000. Blue: Hoechst 33342 staining.| Product Name | Dengue virus NS3 protein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NS3 (Dengue virus) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | DengueVirus1 |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Molecular Weight(MW) | 70 kDa (note) |
|---|
Application
Sample (20 µg of whole cell lysate) A: BHK-21 B: Dengue virus 2 infect BHK-21 C: JEV infect BHK-21 7.5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody diluted at 1:2000. Blue: Hoechst 33342 staining.
Application
Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS3 (Dengue virus 2) antibody at 1:2000 dilution.
Application
NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein by western blot analysis. A. 30 µg BHK-21 whole cell extract B. 30 µg whole cell extract of Dengue virus type 1 infected BHK-21 cells C. 30 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells D. 30 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells E. 30 µg whole cell extract of Dengue virus type 4 infected BHK-21 cells 7.5% SDS-PAGE NS3 (Dengue virus) antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody diluted at 1:2000. Blue: Hoechst 33342 staining.
Application
NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody diluted at 1:2000. Blue: Hoechst 33342 staining.
Application
NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH. Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody diluted at 1:2000. Blue: Hoechst 33342 staining.| Positive Control | Den-2 , Dengue virus type 1 infected BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus type 4 infected BHK-21 , BHK-21 Mock |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
