Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using DENR antibody at 1:500 dilution.Application
DENR antibody detects DENR protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DENR protein stained by DENR antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample (50 ug of whole cell lysate) A: mouse heart 12% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE diluted at 1:2000Application
Immunohistochemical analysis of paraffin-embedded mouse muscle, using DENR antibody at 1:500 dilution.| Product Name | DENR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DENR (density-regulated protein)This gene encodes a protein whose expression was found to increase in cultured cells at high density but not during growth arrest. This gene was also shown to have increased expression in cells overexpressing HER-2/neu proto-oncogene. The protein contains an SUI1 domain. In budding yeast, SUI1 is a translation initiation factor that along with eIF-2 and the initiator tRNA-Met, directs the ribosome to the proper translation start site. Proteins similar to SUI have been found in mammals, insects, and plants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DENR. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Chicken, BovineRat (98%), Zebrafish (85%), Xenopus laevis (90%), Chicken (97%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DRP antibody, DRP1 antibody, SMAP-3 antibody, DENR antibody, density-regulated protein antibody, smooth muscle cell-associated protein 3 antibody, smooth muscle cell associated protein-3 antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) (2) |
Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using DENR antibody at 1:500 dilution.
Application
DENR antibody detects DENR protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DENR protein stained by DENR antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample (50 ug of whole cell lysate) A: mouse heart 12% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE diluted at 1:2000
Application
Immunohistochemical analysis of paraffin-embedded mouse muscle, using DENR antibody at 1:500 dilution.| Positive Control | MCF-7 , mouse heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
