Application
Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE diluted at 1:1000Application
Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Deptor antibody [N1C3] diluted at 1:1000.| Product Name | Deptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DEPDC6 (DEP domain containing 6)Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Deptor. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (97%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DEP.6 antibody, DEPDC6 antibody, DKFZp564B1778 antibody, FLJ12341 antibody, FLJ12428 antibody, FLJ13854 antibody, DEPTOR antibody, DEP domain containing 6 antibody, DEP domain containing mTOR interacting protein antibody, DEP domain-containing mTOR-interac |
|---|---|
| Molecular Weight(MW) | 46 kDa (note) (2) |
Application
Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE diluted at 1:1000
Application
Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Deptor antibody [N1C3] diluted at 1:1000.| Positive Control | A549 , HeLa , HepG2 , HCT116 , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
