Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using Desmocollin 2 antibody at 1:500 dilution.Application
Desmocollin 2 antibody [C1C2], Internal detects Desmocollin 2 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and blotted with Desmocollin 2 antibody [C1C2], Internal diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Desmocollin 2 antibody [C1C2], Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Desmocollin 2 (desmocollin 2)The protein encoded by this gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia-11. Alternative splicing results in two transcript variants encoding distinct isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Desmocollin 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | MouseMouse (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ARVD11 antibody, CDHF2 antibody, DG2 antibody, DGII/III antibody, DKFZp686I11137 antibody, DSC3 antibody, DSC2 antibody, desmosomal glycoprotein II antibody, cadherin family member 2 antibody, desmosomal glycoprotein III antibody, desmocollin-2 antibody, d |
|---|---|
| Target | Desmocollin 2 |
| Molecular Weight(MW) | 100 kDa (note) (2) |
| Cellular Localization | Cell membrane , Cell junction , desmosome |
Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using Desmocollin 2 antibody at 1:500 dilution.
Application
Desmocollin 2 antibody [C1C2], Internal detects Desmocollin 2 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and blotted with Desmocollin 2 antibody [C1C2], Internal diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
