Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Desmoglein 2 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: 293T B: H1299 C: Molt-4 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded DU145 xenograft , using Desmoglein-2 antibody at 1:100 dilution.| Product Name | Desmoglein 2 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Desmoglein 2 (desmoglein-2)Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. This gene product is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Desmoglein 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (87%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ARVC10 antibody, ARVD10 antibody, CDHF5 antibody, CMD1BB antibody, HDGC antibody, MGC117034 antibody, MGC117036 antibody, MGC117037 antibody, DSG2 antibody, cadherin family member 5 antibody, desmoglein-2 antibody, desmoglein 2 antibody |
|---|---|
| Molecular Weight(MW) | 122 kDa (note) |
| Cellular Localization | Cell membrane , Cell junction , desmosome |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Desmoglein 2 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: 293T B: H1299 C: Molt-4 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded DU145 xenograft , using Desmoglein-2 antibody at 1:100 dilution.| Positive Control | 293T , H1299 , Molt-4 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
