Application
Western Blot: DGAT1 Antibody [ - Detection of DGAT1 in HepG2 lysate.Application
Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [ - DGAT1 antibody was tested in Hek293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).| Product Name | DGAT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DGAT1Glucose and insulin are anabolic signals which upregulate the transcriptions of a series of lipogenic enzymes to convert excess carbohydrate into triglycerides for efficient energy storage. DGAT1 is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. DGAT catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. DGAT1 is involved in fat absorption in the intestine and in basal level triglyceride synthesis in adipose tissue, where it is more highly expressed. |
| Immunogen | A synthetic peptide within an internal region (residues 200-300) of the human DGAT1 protein. [Swiss-Prot# O75907] |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Western Blot 2.0 ug/ ml | |
| Species Reactivity | HumanMouseRatZebra FishBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | diacylglycerol Oacyltransferase1, diacylglycerol Oacyltransferase 1, Acyl coenzyme A, ACTrelated gene product 1, diacylglycerol O-acyltransferase 1, Diglyceride acyltransferase, EC2.3.1.20 antibody, DGAT1, ACT-related gene product 1, EC 2.3.1.20 antibody, |
|---|---|
| Target | DGAT1 |
| Tissue Specificity | DGAT1 |
| Cellular Localization | Endoplasmic reticulum; endoplasmic reticulum membrane multi-pass membrane protien. |
Application
Western Blot: DGAT1 Antibody [ - Detection of DGAT1 in HepG2 lysate.
Application
Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [ - DGAT1 antibody was tested in Hek293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).| Application Notes | Western Blot 2.0 ug/ ml |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Storage Buffer | Tris-glycine, 150mM NaCl |
