Application
DGCR8 antibody detects DGCR8 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DGCR8 antibody diluted at a dilution of 1:1000.Application
Immunoprecipitation of DGCR8 protein from Jurkat whole cell extracts using 5 μg of DGCR8 antibody . Western blot analysis was performed using DGCR8 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | DGCR8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DGCR8 (DGCR8 microprocessor complex subunit)This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human DGCR8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus MonkeyMouse (82%), Rat (80%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | pasha , Gy1 , C22orf12 , DGCRK6 , DiGeorge syndrome critical region gene 8 |
|---|---|
| Target | DGCR8 |
| Molecular Weight(MW) | 86 kDa (note) (2) |
Application
DGCR8 antibody detects DGCR8 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DGCR8 antibody diluted at a dilution of 1:1000.
Application
Immunoprecipitation of DGCR8 protein from Jurkat whole cell extracts using 5 μg of DGCR8 antibody . Western blot analysis was performed using DGCR8 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
