Application
Western blot analysis of DGCR8 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). DGCR8 (arrow) was detected using the purified Pab.Application
DGCR8 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DGCR8 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to DGCR8 (Internal)This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 445~475 amino acids from the Center region of human DGCR8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DGCR8, C22orf12, DGCRK6, LP4941, DGCR8, Microprocessor complex subunit DGCR8, DiGeorge syndrome critical region 8, DiGeorge syndrome critical region8 |
|---|---|
| Target | DGCR8 |
| Molecular Weight(MW) | 86.045 kDa (note) |
| Cellular Localization | Colocalizes with nucleolin and RNASEN/DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to spl |
Application
Western blot analysis of DGCR8 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). DGCR8 (arrow) was detected using the purified Pab.
Application
DGCR8 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
