Application
DHCR7 antibody detects DHCR7 protein at cytoplasm in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. DHCR7 antibody diluted at 1:500.Application
DHCR7 antibody detects DHCR7 protein by western blot analysis. HepG2 whole cell extracts (WCE; 30 μg) and membrane extracts (ME; 30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DHCR7 antibody diluted at 1:1000.Application
DHCR7 antibody detects DHCR7 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. DHCR7 antibody diluted at 1:500.| Product Name | DHCR7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DHCR7 (7-dehydrocholesterol reductase)The DHCR7 gene encodes delta-7-sterol reductase (EC 1.3.1.21), the penultimate enzyme of mammalian sterol biosynthesis that converts 7-dehydrocholesterol (7-DHC) to cholesterol. This enzyme removes the C(7-8) double bond introduced by the sterol delta8-delta7 isomerases. In addition, its role in drug-induced malformations is known: inhibitors of the last step of cholesterol biosynthesis such as AY9944 and BM15766 severely impair brain development (Moebius et al., 1998 [PubMed 9465114]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human DHCR7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SLOS , 7-dehydrocholesterol reductase |
|---|---|
| Target | DHCR7 |
| Molecular Weight(MW) | 54 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum membrane |
Application
DHCR7 antibody detects DHCR7 protein at cytoplasm in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. DHCR7 antibody diluted at 1:500.
Application
DHCR7 antibody detects DHCR7 protein by western blot analysis. HepG2 whole cell extracts (WCE; 30 μg) and membrane extracts (ME; 30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DHCR7 antibody diluted at 1:1000.
Application
DHCR7 antibody detects DHCR7 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. DHCR7 antibody diluted at 1:500.| Positive Control | HepG2 (boiled) , HepG2 MF (boiled) , HepG2 (unboiled) , HepG2 MF (unboiled) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
