| Product Name | DHEA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to DehydroepiandrosteroneDehydroepiandrosterone (DHEA) is a major secretory product of the human adrenal cortex during intrauterine development as well as during adulthood. DHEA circulates in the bloodstream as DHEA-sulfate (DHEAS). DHEA has been implicated in a wide range of physiological roles including aging, immunology, learning and memory and obesity. |
| Immunogen | DHEA-15alphaCH2-CO-BSA |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | RIA |
| RIA: Use 0.1 ml diluted antiserum. Reagents: A). 0.1 M Phosphate buffered saline, pH 7.4, containing 1% gelatin and 0.01% sodium azide. B). Dextran coated charcoal suspension: 5 grams of charcoal and 0.5 grams dextran in 1 liter of buffer bath for at least 30 minutes before and during use. RIA Protocol: 1. In polypropylene test tubes add 0.1 ml sample or standard, 0.1 ml tritiated tracer and 0.1 ml diluted antiserum. 2. Vortex the tubes. 3. Allow to equilibrate at room temperature for 30 minutes, then at least 15 minutes at 0¼C in an ice/water bath. 4. Add 1 ml cold dextran charcoal solution, keep in ice-water bath for 10 minutes. 5. Centrifuge at 2000 x g for 10 minutes at 4¼C. 6. Remove supernatant from each tube. Add scintillation fluid to supernatant and determine the amount of radioactivity present in the supernatant. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DHEA |
|---|---|
| Target | DHEA |
| Tissue Specificity | Cross Reactivity:Testosterone |
| Application Notes | RIA: Use 0.1 ml diluted antiserum. Reagents: A). 0.1 M Phosphate buffered saline, pH 7.4, containing 1% gelatin and 0.01% sodium azide. B). Dextran coated charcoal suspension: 5 grams of charcoal and 0.5 grams dextran in 1 liter of buffer bath for at least 30 minutes before and during use. RIA Protocol: 1. In polypropylene test tubes add 0.1 ml sample or standard, 0.1 ml tritiated tracer and 0.1 ml diluted antiserum. 2. Vortex the tubes. 3. Allow to equilibrate at room temperature for 30 minutes, then at least 15 minutes at 0¼C in an ice/water bath. 4. Add 1 ml cold dextran charcoal solution, keep in ice-water bath for 10 minutes. 5. Centrifuge at 2000 x g for 10 minutes at 4¼C. 6. Remove supernatant from each tube. Add scintillation fluid to supernatant and determine the amount of radioactivity present in the supernatant. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Whole antiserum, reconstitute with 11 ml of 0.1 M phosphate buffered saline, pH 7.4, containing 0.1% gelatin and 0.01% sodium azide. |
