Application
Immunoprecipitation of DHFR protein from 293T whole cell extracts using 5 μg of DHFR antibody . Western blot analysis was performed using DHFR antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
DHFR antibody detects DHFR protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12% SDS-PAGE DHFR antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using DHFR antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using DHFR antibody at 1:500 dilution.| Product Name | DHFR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DHFR (dihydrofolate reductase)Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DHFR. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Pig, BovineMouse (88%), Pig (88%), Bovine (85%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DHFRP1 antibody, DYR antibody, DHFR antibody, dihydrofolate reductase antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa (note) (2) |
Application
Immunoprecipitation of DHFR protein from 293T whole cell extracts using 5 μg of DHFR antibody . Western blot analysis was performed using DHFR antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
DHFR antibody detects DHFR protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12% SDS-PAGE DHFR antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using DHFR antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using DHFR antibody at 1:500 dilution.| Positive Control | 293T , A431 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
