Application
Western blot analysis of DHFR (arrow) using rabbit polyclonal DHFR Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DHFR gene.Application
DHFR Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DHFR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of DHFR antibody (C-term) in mouse liver tissue lysates (35ug/lane). KLF4 (arrow) was detected using the purified Pab.| Product Name | DHFR antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to DHFRKey enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. |
| Immunogen | KLH conjugated synthetic peptide between 142~172 amino acids from the C-terminal region of mouse DHFR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Mouse |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Dihydrofolate reductase Dhfr |
|---|---|
| Target | DHFR |
| Molecular Weight(MW) | 21.606 kDa (note) |
Application
Western blot analysis of DHFR (arrow) using rabbit polyclonal DHFR Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DHFR gene.
Application
DHFR Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DHFR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of DHFR antibody (C-term) in mouse liver tissue lysates (35ug/lane). KLF4 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
