Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:10000| Product Name | Dhh antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Dhh (desert hedgehog homolog (Drosophila))This gene encodes a member of the Hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Dhh. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (98%), Rat (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HHG-3 antibody MGC35145 antibody DHH antibody mutant desert hedgehog antibody desert hedgehog homolog antibody desert hedgehog protein antibody desert hedgehog antibody |
|---|---|
| Target | Dhh |
| Molecular Weight(MW) | 44 kDa (note) |
| Cellular Localization | Desert hedgehog protein N-product: Cell membrane; Lipid-anchor; Extracellular side , Desert hedgehog protein C-product: Secreted , extracellular space , Desert hedgehog protein N-product: Cell membrane , Desert hedgehog protein C-product: Secreted , extra |
Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:10000| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
