Application
DIS3 antibody detects DIS3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DIS3 antibody diluted by 1:1000.Application
DIS3 antibody detects DIS3 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DIS3 protein stained by DIS3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
DIS3 antibody detects DIS3 protein at cytosol and nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. DIS3 antibody dilution: 1:500.Application
DIS3 antibody detects DIS3 protein at cytosol and nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DIS3 antibody dilution: 1:500.| Product Name | DIS3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DIS3 (DIS3 mitotic control homolog (S. cerevisiae))Component of the exosome 3'->5' exoribonuclease complex. Required for the 3'-processing of the 7S pre-RNA to the mature nuclear complex. Also associated with the GTPase Ran. Has a 3'-5' exonuclease activity. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human DIS3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | RRP44 EXOSC11 RP11-342J4.3 DIS3 mitotic control homolog (S. cerevisiae) bA555G22.1 KIAA1008 dis3p |
|---|---|
| Target | DIS3 |
| Molecular Weight(MW) | 91 kDa (note) (5) |
| Cellular Localization | Cytoplasm , Nucleus , nucleolus |
Application
DIS3 antibody detects DIS3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DIS3 antibody diluted by 1:1000.
Application
DIS3 antibody detects DIS3 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DIS3 protein stained by DIS3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
DIS3 antibody detects DIS3 protein at cytosol and nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. DIS3 antibody dilution: 1:500.
Application
DIS3 antibody detects DIS3 protein at cytosol and nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DIS3 antibody dilution: 1:500.| Positive Control | U87-MG , SK-N-SH , IMR-32 , SK-N-AS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
