Application
DIS3 Antibody (Center) western blot analysis in mouse bladder tissue lysates (15ug/lane).This demonstrates the DIS3 antibody detected DIS3 protein (arrow).Application
DIS3 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DIS3 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | DIS3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DIS3; KIAA1008; RRP44; Exosome complex exonuclease RRP44; Protein DIS3 homolog; Ribosomal RNA-processing protein 44 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1008 RRP44 |
|---|---|
| Alternative Names | DIS3 KIAA1008 RRP44 Exosome complex exonuclease RRP44 Protein DIS3 homolog Ribosomal RNA-processing protein 44 |
| Molecular Weight(MW) | 109003 Da |
| Function | Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Cytoplasm. Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus. Note=Predominantly located in the nucleus According to PubMed:12429849, found in the nucleolus and according to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3 |
| Entrez Gene | 22894 |
|---|---|
| Nucleotide Accession | NP_001121698.1 NP_055768.3 |
Application
DIS3 Antibody (Center) western blot analysis in mouse bladder tissue lysates (15ug/lane).This demonstrates the DIS3 antibody detected DIS3 protein (arrow).
Application
DIS3 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DIS3 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
