Application
Western blot analysi s of Dkk1 in Lysates from 3T3 cells (Lane 1) and rat kidney (Lane 2).| Product Name | DKK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DKK1Xenopus Dickkopf (Dkk)-1 was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of Dkk have been identified in mammals. Each Dkk molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of Dkk1 and Dkk2 inhibited Wnt-3a-activated signaling, whereas the first Cys-rich domains had no effects. In addition, the second Cys-rich domain of Dkk-2, but not that of Dkk-1, was able to activate the canonical pathway in the presence of LRP6, and this LRP-dependent signaling does not require Dvl. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~35 kDa Dkk1 of human, mouse and rat origins.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKK-1 DKK1 dickkopf1 like dickkopf1 dickkopf homolog 1 dickkopf-1 dickkopf homolog1 SK dickkopf-1 like DKK 1 dickkopf 1 dickkopf 1 like |
|---|---|
| Target | DKK1 |
| Tissue Specificity | This antibody is specific for DKK1. |
Application
Western blot analysi s of Dkk1 in Lysates from 3T3 cells (Lane 1) and rat kidney (Lane 2).| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~35 kDa Dkk1 of human, mouse and rat origins.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
