Application
Immunofluorescence analysis of methanol-fixed A549, using DKK3 antibody at 1:500 dilution.Application
DKK3 antibody [N1C3] detects DKK3 protein at cytoplasm on human normal liver by immunohistochemical analysis. Sample: Paraffin-embedded human normal liver. DKK3 antibody [N1C3] diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000| Product Name | DKK3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DKK3 (dickkopf homolog 3 (Xenopus laevis))This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DKK3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Rhesus Monkey, BovineRat (81%), Dog (87%), Pig (88%), Rhesus Monkey (97%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | REIC antibody RIG antibody DKK3 antibody dickkopf homolog 3 antibody hDkk-3 antibody dickkopf-3 antibody dickkopf-related protein 3 antibody RIG-like 5-6 antibody RIG-like 7-1 antibody dkk-3 antibody regulated in glioma antibody dickkopf homolog |
|---|---|
| Molecular Weight(MW) | 38 kDa (note) (3) |
| Cellular Localization | Secreted |
Application
Immunofluorescence analysis of methanol-fixed A549, using DKK3 antibody at 1:500 dilution.
Application
DKK3 antibody [N1C3] detects DKK3 protein at cytoplasm on human normal liver by immunohistochemical analysis. Sample: Paraffin-embedded human normal liver. DKK3 antibody [N1C3] diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:1000
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000| Positive Control | A549 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
