Application
IP analysis of HeLa cell lysates using control IgG and DLAT antibody. The precipitates were detected by the same DLAT antibody.Application
WB analysis of indicated lysates using DLAT antibody at a dilution of 1:1,000.Application
ICC/IF analysis of HeLa cells using DLAT antibody at a dilution of 1:300.| Product Name | DLAT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [4A4-B6-C10] to DLATThis gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood |
| Immunogen | Purified recombinant human DLAT protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4A4-B6-C10 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| ICC/IF:1:200~1:500 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DLAT DLTA dihydrolipoamide S-acetyltransferase PDC-E2 PDCE2 |
|---|---|
| Target | Pyruvate Dehydrogenase E2 |
| Molecular Weight(MW) | 69kDa (note) |
| Cellular Localization | Mitochondrion matrix. |
Application
IP analysis of HeLa cell lysates using control IgG and DLAT antibody. The precipitates were detected by the same DLAT antibody.
Application
WB analysis of indicated lysates using DLAT antibody at a dilution of 1:1,000.
Application
ICC/IF analysis of HeLa cells using DLAT antibody at a dilution of 1:300.| Application Notes | ICC/IF:1:200~1:500 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol |
