Application
ELISA analysis of control antigen and antigen using DLK1 antibody [3A10].Application
WB analysis of human DLK1 (a.a. 174-349) recombinant protein using DLK1 antibody [3A10].Application
ICC/IF analysis of U251 cells using DLK1 antibody [3A10] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
FACS analysis of NIH3T3 cells using DLK1 antibody [3A10] (green) or a negative control (red).| Product Name | DLK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [3A10] to DLK1This gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass. [provided by RefSeq, Mar 2010] |
| Immunogen | The immunogen corresponding to human DLK1 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3A10 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DLK-1 DELTA1 ZOG PREF1 PG2 FA1 DLK PREF-1 |
|---|---|
| Target | DLK1 |
| Molecular Weight(MW) | 41 kDa (note) |
Application
ELISA analysis of control antigen and antigen using DLK1 antibody [3A10].
Application
WB analysis of human DLK1 (a.a. 174-349) recombinant protein using DLK1 antibody [3A10].
Application
ICC/IF analysis of U251 cells using DLK1 antibody [3A10] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
FACS analysis of NIH3T3 cells using DLK1 antibody [3A10] (green) or a negative control (red).| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
