| Product Name | DMC1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2H12 / 4] to DMC1The DMC1 protein is essential for meiotic homologous recombination. Genetic recombination in meiosis plays an important role in generating diversity of genetic information. The product of this gene is structurally and evolutionary related to the products of the yeast RAD51 and E. coli RecA genes. |
| Immunogen | Recombinant fusion protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 2H12/4 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| For IF: Suggested starting dilution 1:100. Fix cells using a 4% paraformaldehyde solution and permeabilize using SDS (0.03% in a 4% paraformaldehyde solution). Incubate antibody overnight. For IP: Use at an assay dependent dilution. For WB: Suggested starting dilution 1:200. Predicted molecular weight: 41 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatBovine |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | DMC1H HsLim15 11144 Q14565 dJ199H16.1 602721 DMC1 LIM15 |
|---|---|
| Target | DMC1 |
| Tissue Specificity | Specific for DMC1 - does not cross-react with the related protein Rad51. |
| Cellular Localization | Nuclear |
| Application Notes | For IF: Suggested starting dilution 1:100. Fix cells using a 4% paraformaldehyde solution and permeabilize using SDS (0.03% in a 4% paraformaldehyde solution). Incubate antibody overnight. For IP: Use at an assay dependent dilution. For WB: Suggested starting dilution 1:200. Predicted molecular weight: 41 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: PBS |
