Mouse polyclonal to DNA ligase IThere are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase I migrates as a 125kDa phosphoprotein and is implicated in joining Okazaki fragments during DNA synthesis. It also has a role in the base and nucleotide excision repair pathways.
Immunogen
Full-length recombinant human DNA Ligase I protein
Key Feature
Clonality
Monoclonal
Isotype
IgG
Host Species
Mouse
Tested Applications
WB
Optima dilutions/concentrations should be determined by the researcher.:
Species Reactivity
Human
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
126391 MGC130025 P18858 3978 MGC117397 LIG1 DNA ligase I
Target
DNA ligase I
Application
Application
Detection of human DNA Ligase I protein in Raji whole cell lysate using GeneTex polyclonal antiserum
Positive Control
HeLa
Application Notes
Optima dilutions/concentrations should be determined by the researcher.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.