Application
Detection of human Ligase I protein in Raji whole cell lysate using GeneTex DNA Ligase I 10H5 monoclonal antibody Also available in 200ug size The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | DNA ligase I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [10H5] to DNA ligase IThere are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA ligase I migrates as a 125kDa phosphoprotein and is implicated in joining Okazaki fragments during DNA synthesis. It also has a role in the base and nucleotide excision repair pathways. |
| Immunogen | Full-length recombinant human DNA Ligase I protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 10H5 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Predicted molecular weight: 101 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 126391 MGC130025 P18858 3978 MGC117397 LIG1 DNA ligase I |
|---|---|
| Target | DNA ligase I |
| Molecular Weight(MW) | 125 kDa (note) |
| Light Chain Type | kappa |
Application
Detection of human Ligase I protein in Raji whole cell lysate using GeneTex DNA Ligase I 10H5 monoclonal antibody Also available in 200ug size The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | MCF7 |
|---|---|
| Application Notes | For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Predicted molecular weight: 101 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing no preservatives |
