| Product Name | DNA ligase I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [1A9] to DNA Ligase IEukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I. A combination of DNA polymerase epsilon, PCNA, replication factor C, replication protein A, and DNA ligase I is well-suited to the task of creating nucleotide excision repair patches. |
| Immunogen | Full length native protein (purified) (Cow) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1A9 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 101 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | 126391 MGC130025 P18858 3978 MGC117397 LIG1 DNA ligase I MGC139151 |
|---|---|
| Target | DNA ligase I |
| Application Notes | ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 101 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline |
