Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody diluted at 1:500.Application
DNA ligase III antibody detects DNA ligase III protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody diluted by 1:1000.Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using DNA ligase 3 antibody at 1:100 dilution.Application
DNA ligase III antibody detects LIG3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LIG3 protein stained by DNA ligase III antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | DNA ligase III antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNA ligase III (ligase III, DNA, ATP-dependent)This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DNA ligase III. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rhesus Monkey, BovineMouse (92%), Rat (91%), Pig (94%), Rhesus Monkey (97%), Bovine (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LIG2 antibody LIG3 antibody ligase II DNA ATP-dependent antibody polydeoxyribonucleotide synthase [ATP] 3 antibody DNA ligase 3 antibody ligase III DNA ATP-dependent antibody |
|---|---|
| Molecular Weight(MW) | 113 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody diluted at 1:500.
Application
DNA ligase III antibody detects DNA ligase III protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody diluted by 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using DNA ligase 3 antibody at 1:100 dilution.
Application
DNA ligase III antibody detects LIG3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LIG3 protein stained by DNA ligase III antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
