| Product Name | DNA polymerase beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [18S] to DNA Polymerase betaDNA polymerase beta comprises an amino-terminal 8 kDa domain and a carboxy terminal 31 kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyl transferase activity. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulphonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells. |
| Immunogen | Full length protein (Rat). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 18S |
| Host Species | Mouse |
| Tested Applications | ELISAIPWBPLA |
| Immunoprecipitation (denatured): use at a concentration of 2μg / mg. Western Blotting: use at a concentration of 1 - 2μg / ml for 2hrs at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 39 kDa, which corresponds to the predicted molecular weight of DNA Polymerase beta.: |
|
| Species Reactivity | HumanMouseRatHamsterBovine |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | POLB 174760 5423 MGC125976 29240 DNA polymerase beta P06746 |
|---|---|
| Target | DNA polymerase beta |
| Tissue Specificity | Shows no effect on the polymerase activity of beta-pol. |
| Positive Control | HeLa cells |
|---|---|
| Application Notes | Immunoprecipitation (denatured): use at a concentration of 2μg / mg. Western Blotting: use at a concentration of 1 - 2μg / ml for 2hrs at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 39 kDa, which corresponds to the predicted molecular weight of DNA Polymerase beta.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
