| Product Name | DNA polymerase gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to DNA Polymerase gammaAs the only DNA polymerase (pol) present in mitochondria, DNA polymerase gamma (POLG) is necessarily implicated in base excision repair processes to correct oxidative damage to the mitochondrial genome. Therefore, the ability of the catalytic subunit of human POLG has been tested to participate in uracil provoked base excision repair reconstituted in vitro with purified components. Subsequent to actions of uracil DNA glycosylase and apurinic / apyrimidinic endonuclease, human POLG is able to fill a single nucleotide gap in the presence of a 5' terminal deoxyribose phosphate (dRP) flap. The catalytic subunit of human pol gamma catalyzes release of the dRP residue from incised apurinic / apyrimidinic sites to produce a substrate for DNA ligase. Faulty replication of the human mitochondrial genome is thought to be the cause of many diseases. The low selectivity of the mitochondrial DNA polymerase is implicated as the cause of many side effects observed in the treatment of viral infections such as HIV. |
| Immunogen | Purified native human recombinant amino acids 714 - 1061 DNA polymerase gamma |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 5428 POLGA PEO P54098 DNA polymerase gamma 157640 POLG1 SCAE POLG SANDO |
|---|---|
| Target | DNA polymerase gamma |
| Cellular Localization | Mitochondrial |
| Positive Control | HeLa cells |
|---|---|
| Application Notes | For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH7.4 containing 0.2% BSA and 0.09% sodium azide |
