Application
Western Blot: DNA Polymerase iota Antibody - Western blot analysis of DNA Polymerase iota in NIH-3T3 whole cell lysate.Application
Immunocytochemistry/Immunofluorescence: DNA Polymerase iota Antibody - The DNA Polymerase Iota Antibody was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue)| Product Name | DNA polymerase Iota antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to DNA Polymerase Iota ( hRAD30B )DNA polymerase Iota (pol iota, also known as Rad30B) is a novel human polymerase, unlike any others known. It is an extremely low-fidelity enzyme that is highly error-prone when replicating undamaged and damaged DNA in vitro. To date there is, however, no human disease that can be attributed to overexpression or defects in poli. |
| Immunogen | A 15-mer peptide in the C-terminal region of human DNA Polymerase Iota, conjugated to KLH. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB: Use at a dilution of 1/5000 (where a band is seen at ~80 kDa representing DNA Polymerase iota). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | RAD3OB POLI DNA polymerase Iota Q9UNA4 RAD30B 11201 605252 |
|---|---|
| Target | DNA polymerase Iota |
| Tissue Specificity | This antibody is specific for human DNA Polymerase Iota |
Application
Western Blot: DNA Polymerase iota Antibody - Western blot analysis of DNA Polymerase iota in NIH-3T3 whole cell lysate.
Application
Immunocytochemistry/Immunofluorescence: DNA Polymerase iota Antibody - The DNA Polymerase Iota Antibody was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue)| Positive Control | HeLa whole cell extract (100 µg) or GM0193 (lymphoblastoid cell line) |
|---|---|
| Application Notes | WB: Use at a dilution of 1/5000 (where a band is seen at ~80 kDa representing DNA Polymerase iota). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide |
