Application
DNAJB6 antibody [N3C3] detects DNAJB6 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNAJB6 protein stained by DNAJB6 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with DNAJB6 antibody [N3C3] diluted at 1:500.| Product Name | DNAJB6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNAJB6 (DnaJ (Hsp40) homolog, subfamily B, member 6)This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DNAJB6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (94%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DJ4 antibody DKFZp566D0824 antibody DnaJ antibody FLJ42837 antibody HHDJ1 antibody HSJ-2 antibody HSJ2 antibody MGC1152 antibody MGC117297 antibody MRJ antibody MSJ-1 antibody DNAJB6 antibody heat shock protein J2 antibody dnaJ homolog subfami |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) |
| Cellular Localization | Cytoplasm , perinuclear region , Nucleus |
Application
DNAJB6 antibody [N3C3] detects DNAJB6 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNAJB6 protein stained by DNAJB6 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with DNAJB6 antibody [N3C3] diluted at 1:500.| Positive Control | Molt-4 , MDA-MB-231 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
