Application
IP analysis of HeLa lysates using control IgG and DNA-PKcs antibody. The precipitates were detected by the same DNA-PKcs antibody.Application
WB analysis of Molt-4, HL60, K562 and HeLa lysates using DNA-PKcs antibody at a dilution of 1:1,000.Application
ICC/IF analysis of HeLa cells using DNA-PKcs antibody at a dilution of 1:200.| Product Name | DNA-PKcs antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [8D3-B12-F11] to DNA-PKcsThis gene encodes the catalytic subunit of the DNA-dependent protein kinase(DNA-PK).It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination.The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq,Jul 2010] |
| Immunogen | Purified recombinant DNA-PKcs protein fragments expressed in E.coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 8D3-B12-F11 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| ICC/IF:1:200~1:500 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | XRCC7 protein kinase DNA-activated catalytic polypeptide p350 DNPK1 PRKDC HYRC1 HYRC DNAPK |
|---|---|
| Target | DNA-PKcs |
| Molecular Weight(MW) | 450kDa (note) |
| Cellular Localization | Nucleus |
Application
IP analysis of HeLa lysates using control IgG and DNA-PKcs antibody. The precipitates were detected by the same DNA-PKcs antibody.
Application
WB analysis of Molt-4, HL60, K562 and HeLa lysates using DNA-PKcs antibody at a dilution of 1:1,000.
Application
ICC/IF analysis of HeLa cells using DNA-PKcs antibody at a dilution of 1:200.| Application Notes | ICC/IF:1:200~1:500 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal in PBS(pH 7.4)containing with 0.02% sodium azide,50% glycerol. |
