Application
Sample (30 µg of whole cell lysate) A: HCT116 10% SDS PAGE DNASE1L3 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
DNase IL3 antibody [N3C3] detects DNase IL3 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNase IL3 protein stained by DNase IL3 antibody [N3C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Scale bar = 10 μm.| Product Name | DNase IL3 antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNase IL3 (deoxyribonuclease I-like 3)This gene encodes a member of the DNase family. The protein hydrolyzes DNA, is not inhibited by actin, and mediates the breakdown of DNA during apoptosis. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DNase IL3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (83%), Rat (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DHP2 antibody DNAS1L3 antibody LSD antibody DNASE1L3 antibody deoxyribonuclease I-like III antibody DNase gamma antibody DNase I-like 3 antibody Liver and spleen DNase antibody LS-DNase antibody DNase I homolog protein 2 antibody deoxyribonucleas |
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| Molecular Weight(MW) | 36 kDa (note) (3) |
Application
Sample (30 µg of whole cell lysate) A: HCT116 10% SDS PAGE DNASE1L3 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
DNase IL3 antibody [N3C3] detects DNase IL3 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNase IL3 protein stained by DNase IL3 antibody [N3C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Scale bar = 10 μm.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
