Application
IHC analysis of Dnmt1 using at 2 ug/ml on formalin-fixed, paraffin-embedded medullar kidney tissue sectionsApplication
Western blot analysis for Dnmt1 using at 2 ug/ml against 10 µg of cell lysates of mouse ES cell.Application
Western blot analysis of Dnmt1 in human embryonic carcinoma in the A) absence and B) presence of immunizing peptide, recombinant human Dnmt1 protein in the C) absence and D) presence of immunizing peptide, NIH 3T3 (mouse embryonic fribroblast) in the E) absence and F) presence of immunizing peptide, and D3 (mouse embryonic stem cell) in the G) absence and H) presence of immunizing peptide| Product Name | DNMT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [60B1220.1] to DNMT1Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. |
| Immunogen | This antibody was raised against a synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Clone Number | 60B1220.1 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWBChIP |
| For ChIP: Use at 1-2 µg/mL: |
|
| Species Reactivity | HumanMouseZebra Fish |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | DNA (cytosine5 ) methyltransferase1 antibod DNA methyltransferase1 FLJ16293 MGC104992 DNA methyltransferase M.HsaI. DNMT DNMT1 CXXC9 DNA MTase M.HsaI MCMT DNA (cytosine 5 ) methyltransferase 1 antibod DNMT 1 DNA MTase HsaI DNA methyltransfera |
|---|---|
| Target | DNMT1 |
| Tissue Specificity | This can be used in immunohistochemistry at 1-2 ug/ml in human kidney cells. |
Application
IHC analysis of Dnmt1 using at 2 ug/ml on formalin-fixed, paraffin-embedded medullar kidney tissue sections
Application
Western blot analysis for Dnmt1 using at 2 ug/ml against 10 µg of cell lysates of mouse ES cell.
Application
Western blot analysis of Dnmt1 in human embryonic carcinoma in the A) absence and B) presence of immunizing peptide, recombinant human Dnmt1 protein in the C) absence and D) presence of immunizing peptide, NIH 3T3 (mouse embryonic fribroblast) in the E) absence and F) presence of immunizing peptide, and D3 (mouse embryonic stem cell) in the G) absence and H) presence of immunizing peptide| Application Notes | For ChIP: Use at 1-2 µg/mL: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.2% gelatin |
