Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Cross-linked ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or anti-DNMT1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ILIR2.Application
DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm and nucleus in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. DNMT1 antibody [N1], N-term diluted at 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using DNMT1 antibody at 1:200 dilution.Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term diluted at 1:5000.Application
DNMT1 antibody [N1], N-term immunoprecipitates DNMT1 protein in IP experiments. IP samples: HeLa nuclear extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of DNMT1 protein by 4 μg DNMT1 antibody [N1], N-term 5 % SDS-PAGE The immunoprecipitated DNMT1 protein was detected by DNMT1 antibody [N1], N-term diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Product Name | DNMT1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DNMT1 (DNA (cytosine-5-)-methyltransferase 1)DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human DNMT1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AIM antibody CXXC9 antibody DNMT antibody FLJ16293 antibody MCMT antibody MGC104992 antibody DNMT1 antibody DNA (cytosine-5)-methyltransferase 1 antibody CXXC finger protein 9 antibody m.HsaI antibody CXXC-type zinc finger protein 9 antibody DNA |
|---|---|
| Target | DNMT1 |
| Molecular Weight(MW) | 183 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Cross-linked ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or anti-DNMT1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ILIR2.
Application
DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm and nucleus in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. DNMT1 antibody [N1], N-term diluted at 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using DNMT1 antibody at 1:200 dilution.
Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term diluted at 1:5000.
Application
DNMT1 antibody [N1], N-term immunoprecipitates DNMT1 protein in IP experiments. IP samples: HeLa nuclear extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of DNMT1 protein by 4 μg DNMT1 antibody [N1], N-term 5 % SDS-PAGE The immunoprecipitated DNMT1 protein was detected by DNMT1 antibody [N1], N-term diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Positive Control | 293T , A431 , HeLa , HepG2 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
