Application
Immunohistochemistry: Dnmt3b Antibody [ - Dnmt3b antibody was tested in mouse testis using DAB with hematoxylin counterstain.Application
Detection of overexpressed DNMT-38 in HCT116 cells. Lane 1: 40 µg of nuclear extract from untransfected T24 degrees Cells. Lane 2-3: 40 µg of nuclear extracts transiently transfected to overexpress DNMT3B2 and DNMT3B3, respectively.Application
Immunocytochemistry/Immunofluorescence: Dnmt3b Antibody [ - Dnmt3b antibody was tested in NIH-3T3 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).| Product Name | DNMT3B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to DNMT3BInactivation of tumor suppressor genes is a key component of the development of all common forms of human cancers. Aberrant methylation may contribute to many of the changes, both physiological and pathological, associated with aging, including tumor development. Hypermethylation of CpG island-containing promoters is associated with the inactivation of genes important to tumor angiogenesis and progression. DNA methytransferase (DNMT3b) is necessary for the maintenance of the methylation process. |
| Immunogen | This antibody was raised against amino acids 4-101 of the DNMT3b protein. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ICF DNMT3B M.HsaIIIB 242860 1789 Q9UBC3 |
|---|
Application
Immunohistochemistry: Dnmt3b Antibody [ - Dnmt3b antibody was tested in mouse testis using DAB with hematoxylin counterstain.
Application
Detection of overexpressed DNMT-38 in HCT116 cells. Lane 1: 40 µg of nuclear extract from untransfected T24 degrees Cells. Lane 2-3: 40 µg of nuclear extracts transiently transfected to overexpress DNMT3B2 and DNMT3B3, respectively.
Application
Immunocytochemistry/Immunofluorescence: Dnmt3b Antibody [ - Dnmt3b antibody was tested in NIH-3T3 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
