Application
Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: DNMT3B transfected 293T lysates 7.5% SDS PAGE diluted at 1:1000| Product Name | DNMT3B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNMT3B (DNA methyltransferase 3B)Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of mouse DNMT3B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DNA (cytosine-5)-methyltransferase 3B antibody DNA MTase MmuIIIB antibody m.MmuIIIB antibody DNA methyltransferase MmuIIIB antibody MGC124407 antibody Dnmt3b antibody DNA methyltransferase 3B antibody |
|---|---|
| Molecular Weight(MW) | 97 kDa (note) (5) |
Application
Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: DNMT3B transfected 293T lysates 7.5% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
