Application
Immunofluorescence analysis of U20S cell using DNMT3L antibody.Application
Western blot analysis of extracts of various cell lines, using DNMT3L antibody.Application
Immunofluorescence analysis of A549 cell using DNMT3L antibody.| Product Name | DNMT3L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNMT3LCpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012] |
| Immunogen | Recombinant protein of human DNMT3L |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:20 - 1:50, IP 1:20 - 1:50, ChIP 1:20 - 1:50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DNA (cytosine-5-)-methyltransferase 3-like |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) |
Application
Immunofluorescence analysis of U20S cell using DNMT3L antibody.
Application
Western blot analysis of extracts of various cell lines, using DNMT3L antibody.
Application
Immunofluorescence analysis of A549 cell using DNMT3L antibody.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:20 - 1:50, IP 1:20 - 1:50, ChIP 1:20 - 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
