Application
Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE diluted at 1:1000Application
DNMT3L antibody [N1C1] detects DNMT3L protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNMT3L protein stained by DNMT3L antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | DNMT3L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Dnmt3L (DNA (cytosine-5-)-methyltransferase 3-like)CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DNMT3L. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC1090 antibody DNMT3L antibody DNA (cytosine-5)-methyltransferase 3-like antibody cytosine-5-methyltransferase 3-like protein antibody human cytosine-5-methyltransferase 3-like protein antibody DNA (cytosine-5-)-methyltransferase 3-like antibody |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) (3) |
Application
Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE diluted at 1:1000
Application
DNMT3L antibody [N1C1] detects DNMT3L protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNMT3L protein stained by DNMT3L antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
