Rabbit Polyclonal antibody to DPH2 (diphthamide biosynthesis protein 2)This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Immunogen
Recombinant protein encompassing a sequence within the center region of human DPH2. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHC-PWB
*Optimal dilutions/concentrations should be determined by the researcher.
:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
DPH2L2 antibody DPH2 antibody diphthamide biosynthesis-like protein 2 antibody diphthamide biosynthesis protein 2 antibody diphthamide biosynthesis protein 2 homolog-like 2 antibody DPH2-like 2 antibody diptheria toxin resistance protein required for
Molecular Weight(MW)
52 kDa (note) (2)
Application
Application
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded 59T xenograft, using DPH2 antibody at 1:500 dilution.
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.