Application
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using DPPA4 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE diluted at 1:500Application
DPPA4 antibody [N1C3] detects DPPA4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DPPA4 protein stained by DPPA4 antibody [N1C3] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.Application
Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using DPPA4 antibody at 1:400 dilution.Application
Sample (20 ug of whole cell lysate) A: human ESC 12% SDS PAGE diluted at 1:500| Product Name | DPPA4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DPPA4 (developmental pluripotency associated 4)May play a role in maintaining cell pluripotentiality. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DPPA4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 2410091M23Rik antibody FLJ10713 antibody DPPA4 antibody developmental pluripotency-associated protein 4 antibody developmental pluripotency associated 4 antibody |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) (2) |
Application
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using DPPA4 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE diluted at 1:500
Application
DPPA4 antibody [N1C3] detects DPPA4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DPPA4 protein stained by DPPA4 antibody [N1C3] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Application
Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using DPPA4 antibody at 1:400 dilution.
Application
Sample (20 ug of whole cell lysate) A: human ESC 12% SDS PAGE diluted at 1:500| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
