Application
Whole HeLa cell extracts (lane 1) and MCF7 cells (lane 2) were separated on SDS-PAGE and blotted with Rabbit Anti-Debrin and developed with Goat Anti-Rabbit IgG-Alkaline Phosphatase and 5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium substrate| Product Name | Drebrin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to DrebrinThe cortical cytoskeleton, a highly cross-linked submembranal F-actin filament network, plays an important role in cell proliferation, differentiation, migration, cell morphology and oncogenic transformation. Drebrin (developmentally-regulated brain protein) is a major neuronal F-actin binding protein involved in the control of actin dynamics and neuronal morphogenesis. Three major isoforms of drebrin, resulting from alternative splicing of a single gene, have been identified in the brain: the embryonic isoforms designated E1 and E2 and the adult or A isoform (95-120 kDa). An additional truncated form of drebrin A, s-drebrin/A2 (42 kDa), is specifically expressed in the adult brain, but not in non-neuronal tissues. Drebrin is also widely expressed in a variety of cells including epithelial, endothelial, and smooth muscle cells and is associated at cell-cell adhering junction sites. |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 22-42 of Human Drebrin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. Anti-Drebrin recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa) by immunoblotting. An additional unknown band of 20 kDa may be observed in some preparations. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | 1627 DKFZp434D064 Q16643 DBN1 126660 D0S117E Drebrin |
|---|
Application
Whole HeLa cell extracts (lane 1) and MCF7 cells (lane 2) were separated on SDS-PAGE and blotted with Rabbit Anti-Debrin and developed with Goat Anti-Rabbit IgG-Alkaline Phosphatase and 5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium substrate| Application Notes | WB: Use at a dilution of 1/1000. Anti-Drebrin recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa) by immunoblotting. An additional unknown band of 20 kDa may be observed in some preparations. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
