Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DRIL1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
DRIL1 antibody immunoprecipitates DRIL1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of DRIL1 protein by 2 μg of DRIL1 antibody 7.5% SDS-PAGE The immunoprecipitated DRIL1 protein was detected by DRIL1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using ARID3A antibody at 1:100 dilution.Application
DRIL1 antibody [N2C2], Internal detects ARID3A protein by Western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract 7.5 % SDS-PAGE DRIL1 antibody [N2C2], Internal dilution: 1:1000Application
DRIL1 antibody [N2C2], Internal detects ARID3A protein by Western blot analysis. A. 30 µg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE DRIL1 antibody [N2C2], Internal dilution: 1:1000| Product Name | DRIL1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DRIL1 (AT rich interactive domain 3A (BRIGHT-like))This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DRIL1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BRIGHT antibody DRIL1 antibody DRIL3 antibody E2FBP1 antibody ARID3A antibody ARID domain-containing 3A antibody AT rich interactive domain 3A (BRIGHT- like) protein antibody dead ringer-like 1 antibody dead ringer-like protein 1 antibody AT-rich |
|---|---|
| Molecular Weight(MW) | 63 kDa (note) |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DRIL1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
DRIL1 antibody immunoprecipitates DRIL1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of DRIL1 protein by 2 μg of DRIL1 antibody 7.5% SDS-PAGE The immunoprecipitated DRIL1 protein was detected by DRIL1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using ARID3A antibody at 1:100 dilution.
Application
DRIL1 antibody [N2C2], Internal detects ARID3A protein by Western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract 7.5 % SDS-PAGE DRIL1 antibody [N2C2], Internal dilution: 1:1000
Application
DRIL1 antibody [N2C2], Internal detects ARID3A protein by Western blot analysis. A. 30 µg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE DRIL1 antibody [N2C2], Internal dilution: 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
