Rabbit Polyclonal antibody to DroshaMembers of the ribonuclease III superfamily of double-stranded (ds) RNA-specific endoribonucleases participate in diverse RNA maturation and decay pathways in eukaryotic and prokaryotic cells (Fortin et al., 2002 [PubMed 12191433]). The RNase III Drosha is the core nuclease that executes the initiation step of microRNA (miRNA) processing in the nucleus (Lee et al., 2003 [PubMed 14508493]).[supplied by OMIM, Mar 2008]
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Drosha. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
HumanMouseMonkeyRat
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
RNASE3L RNASEN RANSE3L drosha ribonuclease III RN3 ETOHI2 DROSHA HSA242976
Application
Application
Western blot analysis of DROSHA expression in Jurkat (A); HeLa (B); NIH3T3 (C); PC12 (D) whole cell lysates.
Application Notes
WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles.
Storage Buffer
Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.