Application
Western blot of mouse adipose tissue showing specific immunolabeling of the ~25k DsbA-L protein using DsbA-L antibody .| Product Name | DsbA-L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DsbA-LDisulfide-bond-A oxidoreductase-like protein (DsbA-L, previously named as GST Kappa) is an adiponectin-interacting protein. DsbA-L is highly expressed in adipose tissue, and its expression level is negatively correlated with obesity in mice and humans. DsbA-L expression in 3T3-L1 adipocytes is stimulated by the insulin sensitizer rosiglitazone and inhibited by the inflammatory cytokine TNFalpha. Polymorphism of DsbA-L gene has recently been implicated in insulin secretion and body fat distribution. Overexpression of DsbA-L promotes adiponectin multimerization while suppressing DsbA-L expression by RNAi markedly and selectively reduces adiponectin levels and secretion in 3T3-L1 adipocytes. Recent studies identify DsbA-L as a key regulator for adiponectin biosynthesis. |
| Immunogen | Full length recombinant mouse DsbA-L protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Serum |
| Gene Synonyms | GSTkappa Disulfide-bond-A oxidoreductase-like protein GST Kappa DsbA-L DisulfidebondA oxidoreductaselike protein GST-kappa DsbAL DsbA L |
|---|---|
| Molecular Weight(MW) | 25 (note) |
Application
Western blot of mouse adipose tissue showing specific immunolabeling of the ~25k DsbA-L protein using DsbA-L antibody .| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | serum in 40 % glycerol |
