Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DUSP10 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using DUSP10 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela B: HCT116 10% SDS PAGE diluted at 1:1000| Product Name | DUSP10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DUSP10 (dual specificity phosphatase 10)Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization is unique; it is evenly distributed in both the cytoplasm and the nucleus. This gene is widely expressed in various tissues and organs, and its expression is elevated by stress stimuli. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DUSP10. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MKP-5 antibody MKP5 antibody DUSP10 antibody map kinase phosphatase 5 antibody serine/threonine specific protein phosphatase antibody mitogen-activated protein kinase phosphatase 5 antibody dual specificity protein phosphatase 10 antibody dual speci |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) (2) |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DUSP10 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using DUSP10 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela B: HCT116 10% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
